Lycium barbarum Linn. is a well-known salt-tolerant medicinal plant. In this study， the LbALDH3F1 gene was cloned from L. barbarum by RACE， LbALDH3F1 gene promoter sequence was also cloned by PCR. Overexpression vector pCAMBIA 2300 EGFP-LbALDH3F1 was constructed for genetic transformation and subcellular localization in Arabidopsis thaliana， detection of NaCl stress tolerance in transgenic A. thaliana and PCR identification were carried out. The results showed that the LbALDH3F1 was 1700 bp in length， with a CDS of 1446 bp， encoding 481 amino acids. The upstream promoter sequence of LbALDH3F1 was 1850 bp. The secondary structure of LbALDH3F1 was dominated by α-helix and irregular coil. The LbALDH3F1 localized in nucleus and cell membrane. With the severity of NaCl stress， the expression of LbALDH3F1 in L. barbarum showed a tendency of increasing and then decreasing， and the highest expression was found at 200 mmol/L NaCl stress. Genetic transformation of LbALDH3F1 revealed that transgenic LbALDH3F1 A. thaliana was significantly more tolerant to NaCl stress， SOD showed higher activity， proline and chlorophyll a content were significantly increased under 300 mmol/L NaCl stress， whereas H₂O₂ and MDA content were significantly lower than wild type A. thaliana under high salt stress. These results lay a foundation for further studying the function of LbALDH3F1 gene in L. barbarum and its response mechanism in response to salt stress.