Rice direct seeding under waterlogging conditions can not only eliminate the labor cost associating with raising seedlings， transplanting， and drainage， but also effectively prevent the weeds， birds and rats， therefore holding broad application prospects. However， because of the low germination rate under waterlogged conditions， most rice cultivars are unsuitable for direct seeding in flooded fields. Lack of variety seriously limits the development and popularization of waterlogging direct seeding technology. Identifying and screening rice germplasm resources with robust waterlogged seedling characteristics is a crucial foundation for breeding new varieties adapted to waterlogging direct seeding rice. This study utilized rice germplasm materials from domestic and international sources preserved in the Mid-term Crop Germplasm Resource Bank of Hubei province. We measured indicators such as waterlogged seedling rate， germinative force， low-oxygen germination rate， low-oxygen relative germination rate， coleoptile length， low-oxygen coleoptile length， and low-oxygen relative coleoptile length. Seventeen rice germplasm with waterlogged seedling rates exceeding 50% were identified. Correlation analysis revealed significant positive correlations between the waterlogged seedling rate and several indicators， including germinative force， low-oxygen germination rate， coleoptile length， low-oxygen coleoptile length， and low-oxygen relative coleoptile length， with the strongest being between waterlogged seedling rate and low-oxygen coleoptile length was the highest. Thus， the low-oxygen coleoptile length could serve as a vital index for identifying and evaluating the waterlogged seedlings characteristics. By detecting and analyzing the haplotype attribute of OsGF14h， an important regulatory gene for waterlogged seedling characteristics， in 254 rice germplasm resources， 8 germplasm resources with high waterlogged seedling rate （>30%） while OsGF14h gene of non-functional-derived haplotype were identified， corresponding clues for the mining of new genes related to waterlogged seedling characteristics were provided. This study offers valuable insights for improving the efficiency of screening and identification waterlogging tolerant rice germplasm resources， exploring the regulatory genes of waterlogged seedling characteristics， and developing new varieties of waterlogging-tolerant rice suitable for direct seeding.