Genetic Diversity Evaluation of a Rare and Endangered Species Phoebe chekiangensis

doi:10.13430/j.cnki.jpgr.20240618001

2025-4-23- 18

Home > Archive>Volume 26, Issue 3, 2025 >566-574. DOI:10.13430/j.cnki.jpgr.20240618001

Genetic Diversity Evaluation of a Rare and Endangered Species Phoebe chekiangensis
DOI:
                        10.13430/j.cnki.jpgr.20240618001
                    
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                        ZHENG Yongjie1,2ZHENG Yongjie
Camphor Tree Research Institute， Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration， Nanchang 330032;Camphor Tree Research Institute， Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species， Nanchang 330032
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ZHANG Yueting1,2ZHANG Yueting
Camphor Tree Research Institute， Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration， Nanchang 330032;Camphor Tree Research Institute， Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species， Nanchang 330032
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LIU Xinliang1,2LIU Xinliang
Camphor Tree Research Institute， Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration， Nanchang 330032;Camphor Tree Research Institute， Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species， Nanchang 330032
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TU Bailian1TU Bailian
Camphor Tree Research Institute， Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration， Nanchang 330032
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Wu Yanfang1,2Wu Yanfang
Camphor Tree Research Institute， Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration， Nanchang 330032;Camphor Tree Research Institute， Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species， Nanchang 330032
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Affiliation:1.Camphor Tree Research Institute， Jiangxi Academy of Forestry/Camphor Engineering and Technology Research Center of National Forestry and Grassland Administration， Nanchang 330032;2.Camphor Tree Research Institute， Jiangxi Academy of Forestry/Jiangxi Provincial Key Laboratory for Breeding and Efficient Utilization of Jiangxi Native Tree Species， Nanchang 330032
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Fund Project:Foundation projects： Innovation Fund of Jiangxi Province Department of Forestry Research ［（2021）No.15］； Fundamental Research and Talent Development Project of Jiangxi Academy of Forestry （2023522704）

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Abstract:

Phoebe chekiangensis is a rare and endangered species in China， and investigating its genetic diversity and genetic structure of populations provides a scientific basis for the effective protection and utilization. In this study， 15 pairs of SSR markers were used to analyze 175 germplasm resources from 10 populations by capillary electrophoresis. A total of 114 alleles （Na） were detected， with an average of 2.371 effective alleles （Ne） per primer pair. The polymorphic information content （PIC） of the SSR primers ranged from 0.323 to 0.844， averaging 0.629. The populations exhibited a moderate degree of genetic diversity （Ho=0.621， He=0.478）. The inbreeding coefficient within subpopulations （Fis） was -0.237， while the inbreeding coefficient for total population （Fit） was 0.082， indicating a high rate of heterozygosity. There was substantial genetic differentiation （Fst=0.275） and limited gene flow （Nm=0.763） among populations. Analysis of molecular variance （AMOVA） showed that inter-individual differences within populations accounted for 78.7% of the total variation （P<0.001）. Population structure and cluster analysis divided the 10 populations from different provenances into three groups， with some evidence of gene introgression between populations. Habitat fragmentation and human disturbance are likely the primary reasons of endangerment for P. chekiangensis. To protect its genetic diversity of P. chekiangensis， measures by combining in situ conservation and ex situ conservation should be implemented.

Key words:Phoebe chekiangensis;endangered plants;SSR markers;genetic diversity;genetic structure

Reference

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Received:June 18,2024
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