2025-4-25- 2
Home > Archive>Volume 26, Issue 4, 2025 >817-829. DOI:10.13430/j.cnki.jpgr.20240705002
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Combined Transcriptome-Metabolome Analysis of Anthocyanin Synthesis in ‘Zhongyoupan 9’ Peach Fruits under Bagging Condition
Author:
Affiliation:

1.School of Horticulture and Landscape Architecture， Henan Institute of Science and Technology / Henan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plants / Xinxiang Key Laboratory of Germplasm Resources and Genetic Breeding in Pomology， Xinxiang 453003;2.Zhengzhou Fruit Research Institute， Chinese Academy of Agricultural Sciences， Zhengzhou 450000

Fund Project:

Foundation project： Science and Technology Program of Henan Province （222102110440，232102111083， 232102111087）

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    Abstract:

    Bagging-produced flat peaches have gained increasing consumer preference due to their reduced fruit cracking， enhanced visual appeal， and golden to slightly reddish fruit surface. The cultivar ‘Zhongyoupan 9’ shows a distinctive reddish coloration under bagging conditions， attributed to anthocyanin synthesis. This characteristic makes it particularly suitable for bagging cultivation， aligning with consumer preferences for optimal color and appearance. In this study， we investigated the exocarp of bagged fruits of 'Zhongyoupan 9' at 89 and 102 days after blooming （DAFB）， through an integrated approach combining transcriptomic， metabolomic， and bi-histological analyses to elucidate the coloration mechanism. Transcriptome analysis identified 2652 significant differential genes， including 16 genes associated with anthocyanin biosynthesis （PpPAL， PpC4H， Pp4CLs， PpCHSs， PpCHIs， PpF3H， PpF3'H， PpDFR， PpANS， PpUFGT， PpGST）， 56 specifically up-regulated transcription factors， and phytoreceptors PpCRY3， PpUVR8.3. These genes were up-regulated and expressed correlating with anthocyanin content. Metabolomic profiling using high performance liquid tandem mass spectrometry detected 112 significantly differential metabolites， with cyanidin-3，5-O-diglucoside showing the most substantial up-regulation （Log2FC = 16.8）. This metabolite was identified as the primary contributor to the red coloration in the exocarp after bagging. Integrative analysis of transcriptomic and metabolomics data， combined with transcription factor profiling， revealed that PpBL and PpNAC1 up-regulated and activated the anthocyanin regulatory gene PpMYB10.1. This activation promoted the catalytic synthesis of cyanidin-3，5-O-diglucoside by anthocyanin structural genes under bagging conditions， ultimately resulting in the characteristic red coloration of ‘Zhongyoupan 9’. These findings advance our understanding of anthocyanin accumulation patterns in bagged peach fruits and provide a theoretical basis for selecting optimal peach varieties for bagging cultivation practices.

    Reference
    [1] Hara-Kitagawa M,Unoki Y,Hihara S,Oda K. Development of simple PCR-based DNA marker for the red-fleshed trait of a blood peach ‘Tenshin-suimitsuto’. Molecular Breeding,2020,40(1):5
    [2] 王力荣. 我国桃产业现状与发展建议. 中国果树,2021,216(10):1-5Wang L R. Current situation and development suggestions of peach industry in China. China Fruit Trees,2021,216(10):1-5
    [3] 王力荣.中国桃品种改良历史回顾与展望. 果树学报,2021,38(12):2178-2195Wang L R. Historical review and prospect of peach variety improvement in China. Journal of Fruit Science,2021,38(12):2178-2195
    [4] 赵云. 两品种桃外果皮花色苷积累差异及光照调控机制. 杭州:浙江大学,2019Zhao Y. Differences in anthocyanosises' accumulation in peach pericarp of two varieties and light regulation mechanism. Hangzhou:Zhejiang University,2019
    [5] Kapoor L,Simkin A J,George Priya Doss C,Siva R. Fruit ripening:Dynamics and integrated analysis of carotenoids and anthocyanins. BMC Plant Biology,2022,22(1):27
    [6] Li Y X,Lu Y P,Tang D G,Hu B,Zhang Z Y,Wu H W,Fan L J,Cai K W,Tang C,Zhang Y Q,Hong L,Dong J J,Guan B Z,Yin L H,Dai Y,Bai W B,Zheng Z H,Zhu T. Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism. Journal of Translational Medicine,2022,20(1):510
    [7] Zhang Y,Butelli E,Martin C. Engineering anthocyanin biosynthesis in plants. Current Opinion in Plant Biology,2014,19:81-90
    [8] Chen L,Wang X S,Cui L,Li Y B,Liang Y H,Wang S S,Chen Y B,Zhou L,Zhang Y B,Li F. Transcriptome and metabolome analyses reveal anthocyanins pathways associated with fruit color changes in plum(Prunus salicina Lindl.). PeerJ,2022,10:e14413
    [9] Zhao Y,Dong W Q,Wang K,Zhang B,Allan A C,Lin-Wang K,Chen K S,Xu C J. Differential sensitivity of fruit pigmentation to ultraviolet light between two peach cultivars. Frontiers in Plant Science,2017,8:282287
    [10] Ma C Q,Liang B W,Chang B,Yan J Y,Liu L,Wang Y,Yang Y Z,Zhao Z Y. Transcriptome profiling of anthocyanin biosynthesis in the peel of 'Granny Smith' apples(Malus domestica) after bag removal. BMC Genomics,2019,20:353
    [11] Winkel-Shirley B. Flavonoid biosynthesis a colorful model for genetics,biochemistry,cell biology,and biotechnology. Plant Physiology,2001,126(2):485-493
    [12] Tsuda T,Yamaguchi M,Honda C,Moriguchi T. Expression of anthocyanin biosynthesis genes in the skin of peach and nectarine fruit. Journal of The American Society for Horticultural Science,2004,129(6):857-862
    [13] Zhao Y,Dong W Q,Zhu Y C,Allan A C,Wang L K,Xu C J. PpGST1,an anthocyanin-related glutathione S-transferase gene,is essential for fruit coloration in peach. Plant Biotechnology Journal,2020,18(5):1284-1295
    [14] Zhao L,Sun J L,Cai Y M,Yang Q R,Zhang Y Q,Ogutu C O,Liu J J,Zhao Y,Wang F R,He H Q, Zheng B B,Han Y P. PpHYH is responsible for light-induced anthocyanin accumulation in fruit peel of Prunus persica. Tree Physiology,2022,42(8):1662-1677
    [15] 郭少剑.去光条件下葡萄果皮中VvHY5和VvCOP1的转录和蛋白表达分析. 武汉:中国科学院研究生院(武汉植物园),2016Guo S J. Transcription and protein expression analysis of VvHY5 and VvCOP1 in grape peel under light-removing conditions. Wuhan:Graduate University of Chinese Academy of Sciences(Wuhan Botanical Garden),2016
    [16] Guo X,Wang Y T,Zhai Z F,Huang T J,Zhao D,Peng X,Feng C,Xiao Y H,Li T H. Transcriptomic analysis of light-dependent anthocyanin accumulation in bicolored cherry fruits. Plant Physiology and Biochemistry,2018,130:663-677
    [17] Zhu Y F,Su J,Yao G F,Liu H N,Gu C,Qin M F,Bai B,Cai S S,Wang GM,Wang R Z,Shu Q,Wu J. Different light-response patterns of coloration and related gene expression in red pears(Pyrus L.). Scientia Horticulture,2018,229:240-251
    [18] Shi B,Wu H X,Zheng B,Qian M J,Gao A P,Zhou K B. Analysis of light-independent anthocyanin accumulation in mango(Mangifera indica L.). Horticulturae,2021,7(11):423
    [19] Tang B Y,L L,Hu Z L,Chen Y N,Tan T T,Jia Y H,Xie Q L,Chen G P. Anthocyanin accumulation and transcriptional regulation of anthocyanin biosynthesis in purple pepper. Journal of Agricultural and Food Chemistry,2020,68(43):12152-12163
    [20] He Y J,Chen H,Zhou L,Liu Y,Chen H Y. Comparative transcription analysis of photosensitive and non-photosensitive eggplants to identify genes involved in dark regulated anthocyanin synthesis. BMC Genomics,2019,20:1-14
    [21] Hu J T,Chen G P,Zhang Y J,Cui B L,Yin W C,Yu X H,Zhu Z G,Hu Z L. Anthocyanin composition and expression analysis of anthocyanin biosynthetic genes in kidney bean pod. Plant Physiology and Biochemistry,2015,97:304-312
    [22] Wang X Z,Wang Y,Chen B W,Kawabata S,Fang Z Y,Li Y H. Construction and genetic analysis of anthocyanin-deficient mutants induced by T-DNA insertion in 'Tsuda' turnip(Brassica rapa). Plant Cell,Tissue and Organ Culture,2017,131:431-443
    [23] Yang J F,Chen Y Z,Saneyuki K,Li Y H,Yu W. Identification of light-independent anthocyanin biosynthesis mutants induced by ethyl methane sulfonate in turnip "Tsuda"(Brassica rapa). International Journal of Molecular Sciences,2017,18(7):1288
    [24] Huang H,Li Y,Dai S. Investigation of germplasm in chrysanthemum cultivars with light-independent coloration. Acta Horticulturae,2016,1185(9):55-64
    [25] Nakatsuka A,Yamagishi M,Nakano M,Tasaki K,Kobayashi N. Light-induced expression of basic helix-loop-helix genes involved in anthocyanin biosynthesis in flowers and leaves of Asiatic hybrid lily. Scientia Horticulturae,2009,121(1):84-91
    [26] Hu W,Figueroa-Balderas R,Chi-Ham C,Lagarias J C. Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B. Plant Direct,2020,4(4):e00210
    [27] Yao G F,Ming M L,Allan A C,Gu C,Li,L T,Wu X,Wang R Z,Chang Y J,Qi K J,Zhang S L,Wu J. Map‐based cloning of the pear gene MYB 114 identifies an interaction with other transcription factors to coordinately regulate fruit anthocyanin biosynthesis. The Plant Journal,2017,92(3):437-451
    [28] Daccord N,Celton J,Linsmith G,Becker C,Choisne N,Schijlen E,Geest H,Bianco L,Micheletti D,Velasco R,Erica Adele Di Pierro E,Gouzy J,D J G,Guérif P,Muranty H,Durel C,Laurens F,Lespinasse Y,Gaillard S,Aubourg S,Quesneville H,Weigel D,Weg E,Troggio M,Bucher E. High-quality de novo assembly of the apple genome and methylome dynamics of early fruit development. Nature Genetics,2017,49(7):1099-1106
    [29] Fraga C G,Clowers B H,Moore R J,Zink E M. Signature-discovery approach for sample matching of a nerve-agent precursor using liquid chromatography-mass spectrometry,XCMS,and chemometrics. Analytical Chemistry,2010,82(10):4165-4173
    [30] Zhao L,Zhang Y Q,Sun J L,Yang Q R,Cai Y M,Zhao C P,Wang F R,He H P,Han Y P. PpHY5 is involved in anthocyanin coloration in the peach flesh surrounding the stone. The Plant Journal,2023,114(4):951-964
    [31] Zhou H,Wang K L,Wang H L,Gu C,Dare A P,Espley R V,He H P,Allan A C,Han Y P. Molecular genetics of blood‐fleshed peach reveals activation of anthocyanin biosynthesis by NAC transcription factors. The Plant Journal,2015,82(1):105-121
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  • Received:July 05,2024
  • Online: April 03,2025
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