Sepal Phenotypic Evaluation of 200 Pear Germplasm Resources
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1.National Key Laboratory for Germplasm Innovation &2.Utilization of Horticultural Crops&#;3.Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Sciences;4.Zhongyuan Research Center,Chinese Academy of Agriculture Sciences;5.Chuxiong Yunfruit Industry Technology Research Institute;6.College of Horticulture and Landscape Architecture,Henan Institute of Science and Technology

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the Key Research and Development Project of Henan Province (241111113500);the Major Science and Technology Project of Yunnan Province (202302AE090005);the National Natural Science Foundation of China (32202439);the Major Science and Technology Projects of Henan Province (221100110400);the Earmarked Fund for China Agriculture Research System (CARS-28);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-ASTIP);the Henan Province Science and Technology Research Project (232102110180);the International Science and Technology Cooperation Project of Henan Province (242102521063).

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    Abstract:

    In order to understand the diversity of sepal traits in different pear germplasm resources and analyze the effect of sepals on fruit appearance, the diversity analysis, correlation analysis and cluster analysis of sepal-related traits of 200 pear germplasm resources were carried out in this study. The results showed that the number of pear germplasm resources with extremely high and low calyx abscission rates was significantly higher than that in other groups, and the number of germplasm resources with different calyx abscission rates showed a U-shaped distribution. For the germplasm resources of sepal abscission, the sepal development process was divided into three stages: before the formation of abscission zone (2-4 days after flowering), during the formation period of abscission zone (5-7 days after flowering), and after the formation of abscission zone (8-10 days after flowering). Among the germplasm resources of persistent calyx and residue calyx, the calyx was mainly exhibited “aggregation” and “upright” posture. In Oriental Pear, calyx-shed fruit generally has no calyx convex, and the calyx convex on the persistent calyx fruit mostly affects the fruit shape. Phenotypic measurement, data analysis and comprehensive evaluation of sepals showed that the thickness and area of sepals varied greatly among different germplasm resources, but had no direct effect on fruit shape. In European Pear, sepals are generally thin and small, and have little effect on fruit shape. The analysis results of sepal persistence on the abnormal fruit showed that 33 varieties of persistent calyx had different degrees of abnormal fruit. The abnormal fruit of persistent calyx fruit mainly included two types: oblique fruit and protruding calyx fruit, while the abnormal fruit in residual calyx fruit was oblique fruit, indicating that the existence of sepal would affect the fruit shape to a certain extent, but it was not the only factor. Correlation analysis showed that the calyx abscission rates were significantly negatively correlated with the calyx convexity, the higher the calyx abscission rates, the less obvious of the calyx convexity. The 200 pear germplasm resources were mainly divided into four categories by cluster analysis, with calyx shedding rate as the main feature (80.7 % - 100.0 %, 16.6 % - 40.2 %, 45.5 % - 78.5 %, 0.00 % - 11.9 %). The results provide an important reference for the utilization of pear germplasm resources and variety breeding.

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History
  • Received:October 25,2024
  • Revised:November 26,2024
  • Adopted:December 27,2024
  • Online: January 22,2025
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