The horizontal resistance to potato late blight is controlled by minorgene with low efficiency through phenotype evaluation. In order to innovate potato germplasm resources with horizontal resistance, this study selected 8 parental materials for cross and constructed 9 hybrid populations. Combined with the late blight resistance evaluation by detached leaf assay, molecular assisted selection was performed on 60 resistant and susceptible hybrid offspring materials by horizontal resistance gene PCR sequencing, 19 single nucleotide polymorphism (SNP) sites of 8 resistance genes were detected. The results showed that StAOS2-SNP691、StAOS2-SNP692、StGP28-SNP794、PLOX1-SNP8344 beneficial SNP loci was positively associated with late blight resistance. the proportion of beneficial SNP homozygous sites in disease resistant materials in the hybrid offspring population was 9.56%, which was higher than that in susceptible materials in the offspring population (5.65%) and higher than that in parental materials (7.89%). A phylogenetic analysis of 19 SNP markers in 8 genes cluster 60 materials into 3 resistant branches and 3 susceptible branches. By combining phenotype, breeding pedigree and molecular markers of horizontal resistance genes, breeding values related to resistance to late blight were calculated, and excellent individual plants such as PC2312-3, PC2312-15, and PC2313-19 with high breeding values and beneficial SNP homozygous loci were screened, providing materials for genetic improvement of potato horizontal resistance populations.