The production technology of sweet potato virus-free seedlings by directly breeding field seedlings from virus-free tissue culture seedlings or minituber seedlings has become a new promotion and application technology. In order to investigate the differences between tissue culture seedlings and minituber seedlings of different sweetpotato varieties.on field growth, yield and quality. 12 sweetpotato varieties of tissue culture seedling and minituber seedlings were used as experimental materials. The results show that: (1) Photosynthetic characteristics, Mingmenjinshi, Shenglibaihao, Ningzishu 4 and Pushu 32 tissue culture seedlings of photosynthetic activity and pigment content were higher than minituber seedling, while Shangshu 19 tissue culture seedlings were lower than minituber seedlings. (2) Fresh potato yield, Mingmenjinshi, Shenglibaihao and Ningzishu 4 tissue culture seedlings was respectively higher than minituber seedlings by 30.95%, 21.90% and 14.67%, but Shangshu 19 tissue culture seedlings was lower than minituber seedlings by 10.42%. (3) Fresh potato quality, Ningzishu 4, Hongyao, Mashali and Pushu 32 tissue culture seedlings of fresh potato (crude starch, soluble sugar, crude protein) were lower than minituber seedlings. Jinhaimeixiu tissue culture seedlings of crude starch was higher than minituber seedlins, but tissue culture seedlings of reducing sugar and soluble sugar were lower than minituber seedlings. Mingmenjinshi tissue culture seedlings of dry matter rate were higher than minituber seedlings. (4) The correlation analysis results of photosynthesis, yield and quality of virus-free seedlings of sweetpotato varieties showed that，only the water utilization rate and dry matter rate were negatively correlated of tissue culture seedlings, while the net photosynthetic rate, water utilization rate, carboxylation efficiency had significant effects on the fresh potato quality of minituber seedlings. This study can provide a theoretical basis for the efficient utilization of tissue culture seedlings and minituber seedlings of different sweet potato varieties.