Glutamate receptor like channels (GLRs) are a type of Ca2+channel proteins involved in cellular metabolism, seed germination, plant growth and development, as well as long-distance signal transmission. Here, in this study, a total of 29 soybean GLR members were identified through a comprehensive proteomic identification of soybean. Their phylogenetic relationship suggests that these soybean GLR genes can be classied into three subfamilies, members within the same subfamily exhibits conserved gene structure and motif compositions. Cis-acting elements analysis indicates that the promoter regions of soybean GLRs contain a variety of regulatory elements related to hormones, stress, light, and plant growth and development. The analysis of tissue and stress expression patterns shows that GLR genes are highly expressed across various tissues in soybeans and involved in salt and alkaline stress. GmGLRs exhibit significant variations under salt and alkaline stress by qRT-PCR analysis, suggesting that GmGLR3.13/15/4.8/4.9/4.10 may play an important role in stress response.In summary, the results of this study provide an important theoretical basis for further investigating the role of soybean GLR genesin stress responses.