2025-6-11- 21
  • Home
  • About Journal
  • Editorial Board
  • Author
    Instruction
    Copyright Agreement
  • Ethcis Statement
  • Subscribe
  • Contact
  • 中文
Home > Archive>Volume 0, Issue 9, >. DOI:10.13430/j.cnki.jpgr.20250118002 Online First
PDF HTML XML Export Cite reminder
Transcriptome analysis of embryonic and non-embryonic callus of peanut
DOI:
10.13430/j.cnki.jpgr.20250118002
CSTR:
Author:
  • WU Ri-lian 1,2

    WU Ri-lian

    Institute of Cotton Research,Shanxi Agricultural University;College of Agriculture,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • SHANG-GUAN Xiao-xia 1

    SHANG-GUAN Xiao-xia

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • WU Cui-cui 1

    WU Cui-cui

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • XIA Zhi 1

    XIA Zhi

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • ZHANG Xiao-ling 1

    ZHANG Xiao-ling

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • LI Huan-li 1

    LI Huan-li

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • LAN Gang 1

    LAN Gang

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • PAN Zhuan-xia 1

    PAN Zhuan-xia

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • HOU Bao-guo 1

    HOU Bao-guo

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • LV Be-ibei 1

    LV Be-ibei

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
  • LI Peng-bo 1,2

    LI Peng-bo

    Institute of Cotton Research,Shanxi Agricultural University
    Find this author on All Journals
    Find this author on BaiDu
    Search for this author on this site
Affiliation:

1.Institute of Cotton Research,Shanxi Agricultural University;2.College of Agriculture,Shanxi Agricultural University

Clc Number:

Fund Project:

Reveal the list and take command project of Shanxi Provincial Major Science and Technology programs (202201140601025-4-03) ; Technology innovation enhancement project of Shanxi Agricultural University (CXGC2023054) ; Doctoral fund project of institute of cotton research, Shanxi Agricultural University (SBSJJ2023-02)

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference [49]
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Peanut (Arachis hypogaea L.) is the fourth largest oil crop in the world. Tissue culture is an important procedure in genetic transformation of peanut. However, the differentiation of peanut callus is restricted by genotype. In this study, Luohua 22, a cultivar with strong callus differentiation ability, was screened from 9 germplasm resources through establishing a peanut genetic transformation system. Transcription sequencing analyses were carried out using T1 callus (embryogenic callus, easy to differentiate into seedlings), T2 callus (non-embryogenic callus, difficult to differentiate into seedlings) during the differentiation process of Luohua 22, and callus at 0 day of differentiation was set as control (CK). Compared with CK, there were 1792 and 868 differentially expressed genes (DEGs) in T1 and T2 types of callus, respectively. The GO functional enrichment analysis showed that the DEGs in T1 type of callus were mainly enriched in the pathways of meristematic organization and stem cell population maintenance, while those in T2 type of callus were mainly enriched in the pathways of phenylpropane biosynthesis and metabolic pathway. Protein family analysis showed that DEGs in T1 and T2 type callus contained 2459 TFs, among which the cytochrome P450 family was significantly enriched. By Protein-protein interaction network (PPI) analysis, five key genes, i.e., AhAE3ZZG, AhP17M1H, AhA6R79F, AhZFZ3ZQ and AhHMN99B, were mined, which might play important roles in promoting peanut callus differentiation. The results of this study would provide a scientific basis for furhter exploring the key genes affecting the differentiation process of peanut cotyledon node callus, and the subsequent analysis of the molecular mechanism of peanut callus differentiation to form regenerated plants.

    Key words:peanut; cotyledon node; callus differentiation; RNA-Seq; Protein genes
    Reference
    Zhang H, Tang Y Y, Yue Y L, Chen Y. Advances in the evolution research and genetic breeding of peanut. Gene. 2024, 916: 148425.
    Song Z Y, Tian J L, Fu W Z, Li L, Lu L H, Zhou L, Shan Z H, Tang G X, Shou H X. Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability. Journal of Zhejiang University-SCIENCE B. 2013, 14 (4): 289-298.
    Nivya V M, Shah J M. Recalcitrance to transformation, a hindrance for genome editing of legumes. Frontiers in Genome Editing. 2023, 5: 1247815.
    Qiu L, Su J, Fu Y, Zhang K. Genetic and transcriptome analyses of callus browning in chaling common wild rice (Oryza rufipogon Griff.). Genes. 2023, 14 (12): 2138.
    张晓玲,龙芸,葛飞,管中荣,张晓祥,王艳丽,沈亚欧,潘光堂.玉米幼胚胚性愈伤组织再生能力相关性状遗传研究.遗传, 2017, 39 (02): 143-155.
    Zhang X L, Long Y, Ge F, Guan Z G, Zhang X X, Wang Y L, Shen Y , Pan G T. Genetic study on traits related to callus regeneration ability of maize embryonic callus. Hereditas, 2017, 39 (02): 143-155.
    Kumar S, Ruggles A, Logan S, Mazarakis A, Tyson T, Bates M, Grosse C, Reed D, Li Z, Grimwood J, Schmutz J, Saski C. Comparative transcriptomics of non-embryogenic and embryogenic callus in semi-recalcitrant and non-recalcitrant upland cotton lines. Plants. 2021, 10 (9): 1775.
    Limbua Purity G,Ngugi Mathew P,Oduor Richard O. Genetic transformability of selected Kenyan groundnut (Arachis hypogaea L.) genotypes with IPT gene using cotyledonary node explants. Advances in Agriculture, 2022, Article ID 2516843, https://doi.org/10.1155/2022/2516843.
    卢春生,廖福琴,林芸.不同组培方式对花生子叶离体组培获得再生苗影响的研究.山西农业大学学报(自然科学版), 2014, 34 (03): 245-248.
    Lu C S, Liao F Q, Lin Y. Study on the effect of different tissue culture methods on the acquisition of regenerated seedlings from In vitro tissue culture of peanut cotyledons. Journal of Shanxi Agricultural University (Natural Science Edition), 2014, 34 (03): 245-248.
    刘北东,朱延明,李海燕,杨谦.大豆子叶节再生影响因素的研究.大豆科学,2002,(02):88-92.
    Liu B D, Zhu Y M ,Li H Y, Yang Q. Study on the influencing factors of cotyledon regeneration in soybean. Soybean Science, 2002, (02): 88-92.
    Luo D D, Shi L, Sun Z Q, Qi F Y, Liu H F, Xue L L, Li X N, Liu H, Qu P Y, Zhao H H, Dai X D, Dong W Z, Huang Z B, Fu L Y, Zhang X Y. Genome-wide association studies of embryogenic callus induction rate in peanut ( Arachis hypogaea L.). Genes, 2024, 15 (2):160
    Müller-Xing R, Xing Q. The plant stem-cell niche and pluripotency: 15 years of an epigenetic perspective.Frontiers in Plant Science. 2022, 13: 1018559.
    Chen B, Maas L, Figueiredo D, Zhong Y, Reis R, Li M, Horstman A, Riksen T, Weemen M, Liu H, Siemons C, Chen S, Angenent G C, Boutilier K. BABY BOOM regulates early embryo and endosperm development. Proceedings of the National Academy of Sciences of USA. 2022, 119 (25): e2201761119.
    Zhang X, Wang Y, Yan Y, Peng H, Long Y, Zhang Y, Jiang Z, Liu P, Zou C, Peng H, Pan G, Shen Y. Transcriptome sequencing analysis of maize embryonic callus during early redifferentiation. BMC Genomics. 2019, 20 (1): 159.
    Cheng Z J, Wang L, Sun W, Zhang Y, Zhou C, Su Y H, Li W, Sun T T, Zhao X Y, Li X G, Cheng Y, Zhao Y, Xie Q, Zhang X S. Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3. Plant Pbysiology. 2013, 161 (1): 240-51.
    贾思年,魏倩涵,苗蓉,彭云玲,刘允军.玉米愈伤组织形成的转录组分析.植物遗传资源学报, 2023, 24 (04): 1151-1160.
    Jia S N, Wei Q H, Miao R, Peng Y L, Liu Y J. Transcriptome analysis of callus formation in maize. Journal of Plant Genetic Resources, 2023, 24 (04): 1151-1160.
    葛梦杰,张朝军,栗战帅,王小艳,孙志茂,陈柏志,张迁,乔凯凯,马常凯,曹晓聪,吕娇艳,王龙,王艳文,张茹佳,聂君伟,马启峰,陈全家,范术丽.红光促进棉花胚性愈伤诱导的转录组分析.分子植物育种, 2024, 22 (06):1774-1783.
    Ge M J, Zhang C J, Li Z S, Wang X Y, Sun Z M, Chen B Z, Zhang Q, Qiao K K, Ma C K, Cao X C, Lv J Y, Wang L, Wang Y W, Zhang R J, Nie J W, Ma Q F, Chen Q J, Fan S L. Transcriptome analysis of red light-promoted embryogenic callus induction in cotton. Molecular Plant Breeding, 2024, 22 (06): 1774-1783.
    楚宗丽,张睿男,李亮杰,等.小麦WRKY转录因子的鉴定及其在胚性愈伤组织形成中的表达分析.麦类作物学报, 2021, 41 (12):1469-1478.
    Chu Z L, Zhang R N, Li L J, Sun J Y, Wang F J, Zhou Q, Tong S L. Identification of WRKY transcription factors in wheat and their expression analysis in embryogenic callus formation. Journal of Triticeae Crops, 2021, 41 (12): 1469-1478.
    Jiao Y, Tan J, Guo H, Huang B, Ying Y, Ramakrishnan M, Zhang Z. Genome-wide analysis of the KNOX gene family in moso bamboo: insights into their role in promoting the rapid shoot growth. BMC Plant Biology. 2024, 24 (1): 213.
    Luo Y, Hu B, Ji H, Jing Y, Zhang G, Yan Y, Yang B, Peng L. Sequence characteristics, expression and subcellular localization of PtCYP721A57 gene from cytochrome P450 family in Polygala tenuifolia willd. PeerJ. 2024, 12: e18089 https://doi.org/10.7717/peerj.18089.
    Zhou M, Jiang Y, Liu X, Kong W, Zhang C, Yang J, Ke S, Li Y. Genome-wide identification and evolution analysis of the CYP76 subfamily in rice (Oryza sativa). International Journal of Molecular Sciences. 2023, 24 (10): 8522.
    Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nature Biotechnology. 2020, 38 (11): 1274-1279.
    Xue Q, Zhang X, Yang H, Li H, Lv Y, Zhang K, Liu Y, Liu F, Wan Y. Transcriptome and metabolome analysis unveil anthocyanin metabolism in pink and red testa of peanut (Arachis hypogaea L.). International Journal of Genomics. 2021:5883901.
    .商二磊,阿曼古力.海瓦尔,孙博.拟南芥花分生组织活性调控机制的研究进展.中国科学:生命科学, 2022, 52 (03): 336-346.
    Shang E L, Amangul H W, Sun B. Research progress on the regulatory mechanism of meristem activity in arabidopsis thaliana. Science China Life Sciences, 2022, 52 (03): 336-346.
    Zheng Q, Zheng Y, Ji H, Burnie W, Perry S E. Gene regulation by the AGL15 transcription factor reveals hormone interactions in somatic embryogenesis. Plant Pbysiology. 2016 172 (4): 2374-2387
    Bharathan G, Goliber T E, Moore C, Kessler S, Pham T, Sinha N R. Homologies in leaf form inferred from KNOXI gene expression during development. Science. 2002, 296 (5574): 1858-1860.
    Tsuda K, Maeno A, Otake A, Kato K, Tanaka W, Hibara K I, Nonomura K I. YABBY and diverged KNOX1 genes shape nodes and internodes in the stem. Science. 2024, 384 (6701): 1241-1247.
    Li R, Wei Z, Li Y, Shang X, Cao Y, Duan L, Ma L. Ski-interacting protein interacts with shoot meristenless to regulate shoot apical meristem formation. Plant Pbysiology. 2022, 189 (4): 2193-2209.
    Lee K, Park OS, Seo PJ.ArabidopsisATXR2 deposits H3K36me3 at the promoters ofLBDgenes to facilitate cellular dedifferentiation. Science Signaling. 2017, 10(507):DOI: 10.1126/scisignal.aan0316.
    Bull T, Debernardi J, Reeves M, Hill T, Bertier L, Van Deynze A, Michelmore R. GRF-GIF chimeric proteins enhance in vitro regeneration and Agrobacterium-mediated transformation efficiencies of lettuce (Lactuca spp.). Plant Cell Reports. 2023, 42 (3): 629-643.
    聂天巡,国钰环,彭正松,杨在君.EPF/EPFL家族基因调控植物生长发育和非生物胁迫响应的研究进展.生命科学, 2024, 36 (08): 1012-1019.
    Nie T X, Guo Y H, Peng Z S, Yang Z J. Research progress on the regulation of plant growth and development by EPF/EPFL family genes in response to abiotic stress. Life Sciences, 2024, 36 (08): 1012-1019.
    Wu M, Wang Y, Zhang S, Xiang Y. A LBD transcription factor from moso bamboo, PheLBD12, regulates plant height in transgenic rice. Plant Molecular Biology. 2024, 114 (5): 95.
    Wu C, Hou B, Wu R, Yang L, Lan G, Xia Z, Cao C, Pan Z, Lv B, Li P. Genome-wide analysis elucidates the roles of AhLBD genes in different abiotic stresses and growth and development stages in the peanut (Arachis hypogea L.). International Journal of Molecular Sciences. 2024, 25 (19): 10561.
    胡雅丹,伍国强,刘晨,魏明.MYB转录因子在调控植物响应逆境胁迫中的作用.生物技术通报, 2024, 40 (06): 5-22.
    Hu Y D, Wu G Q, Liu C, Wei M. Role of MYB transcription factors in regulating plant response to stress. Biotechnology Bulletin, 2024, 40 (06): 5-22.
    Li Z, Gao J, Wang G, Wang S, Chen K, Pu W, Wang Y, Xia Q, Fan X. Genome-wide identification and characterization of GASA gene family in Nicotiana tabacum. Frontiers in Genetics. 2022, 12: 768942.
    Kai W ,Yanshu Q ,Hao R ,Xin H, Yating T, Lian X.Identification and expression analysis of the populus trichocarpa GASA-Gene family.International Journal of Molecular Sciences,2022,23 (3): 1507-1507.
    He J, Chen Q, Xin P, Yuan J, Ma Y, Wang X, Xu M, Chu J, Peters RJ, Wang G. CYP72A enzymes catalyse 13-hydrolyzation of gibberellins. Nature Plants. 2019, (10): 1057-1065.
    Kiba T, Mizutani K, Nakahara A, Takebayashi Y, Kojima M, Hobo T, Osakabe Y, Osakabe K, Sakakibara H. The trans-zeatin-type side-chain modification of cytokinins controls rice growth. Plant Pbysiology. 2023, 192 (3): 2457-2474.
    党媛玥,马建江,杨书贤,宋吉坤,贾冰,冯盼,陈全家,于霁雯.棉花 β-tubulin基因家族的鉴定及其在纤维发育中的表达.中国农业科学, 2023, 56 (23): 4585-4601.
    Dang Y Y, Ma J J, Yang S X, Song J K, Jia B , Feng P , Chen Q J, Yu J W. Identification of β-tubulin gene family and its expression in fiber development in cotton. Scientia Agricultura Sinica, 2023, 56 (23): 4585-4601.
    Wang C, Xiang Y, Qian D. Current progress in plant V-ATPase: from biochemical properties to physiological functions. Journal of Plant Physiology. 2021, 266: 153525.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Related Videos

Share
0
Article Metrics
  • Abstract:46
  • PDF: 86
  • HTML: 0
  • Cited by: 0
History
  • Received:January 18,2025
  • Revised:April 08,2025
  • Adopted:April 16,2025
  • Online: April 22,2025
  • Published:
Article QR Code
You are the 675625th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.