Abstract:To illustrate the regulation mechanism of parthenocarpy and the genes controlling this process, as well as provide basic materials in eggplant parthenocarpy breeding, the parthenocarpy/non parthenocarpy near isogenic lines were constructed in this study. Two groups of near isogenic lines (NILs) JDX1 and JDX8 were generated by hybridization, backcross and selfing, in combination with parthenocarpy identification and agronomic traits investigation. 219 pairs of SSR primers covering eggplant genome were used to genotype eggplant NILs and their parents. JDX1 and JDX8 showed 97.14% and 88.23% on the genetic similarity with respective reincarnation parents, respectively. The parthenocarpy identification showed that, in difference with recurrent parents, JDX1 and JDX8 had been completely transformed into non parthenocarpic lines. There were no significant differences in plant height, first flower height, node spacing, leaf length and width, fruit length and thickness between NILs and their recurrent parents. By considering the agronomic characteristics such as fruit color and strain type, JDX1 and JDX8 showed higher similarity/identity with the reincarnation parent. Collectively, the success on creation of eggplant parthenocarpy lines laid a foundation for the basic research of single solidity.

Abstract:Astragalus sinicus L. is one of cross-pollinated plants with heterogeneity in cultivar population which results in difficulty for cultivar identification. In this study, 3 cultivars selected from Minzi cultivar series bred in Fujian have been analyzed by using SSR molecular marker and the effect of sampling strategy on cultivar differentiation was investigated. The results showed that: (1) When the primer combination was confirmed by 4 pairs and the sampling amount ranged from 5 to 50 in each cultivar, the amplified loci and the observed number of alleles tended to increase in the cultivar, but the effective number of alleles，Shannon index，and genetic diversity index tended to decline after reaching to a maximum as sampling amount was as much as 30 for total sample; With the increase of the amount of sample, the Nei’s genetic distance between cultivars and the PhiPT value among population obtained from AMOVA reduced，but the confidence level of PhiPT was raised. (2) When the sample capacity was constant with 30 or 50, and the primer number was increased to 6 with the added 2 primers amplifing the marking locus of different frequency among the cultivars, the tested cultivars could be effectively identified because of the higher PhiPT value and more significant confidence level (P<0.001). The analysis of principal component further indicated that the distribution of samples from 3 tested cultivars along principal component axis was similar between sampling amount of 30 and 50 . This study of sampling strategy for Astragalus sinicus indicated that: In order to enhance the ability in identification of cultivar of Astragalus sinicus L., the optimum sampling amount was as much as 30 that could achieve the better effect for cultivar identification as well as reduce the cost in analysis.